PP-110 Complementary foods in case of allergy: sensitization and intestinal microbiota

Aim To determine associations between specific sensitization and featurе of the intestinal microbiota composition in children with food allergy during nutrition diversification. Material and Method This is a cross-sectional, single-center study of 90 children with food allergy in groups: 6–12 (I, n=56) and 13–18 months old (II, n=34). The levels of total IgE (TIgE) and sIgE were studied using ALEX2 allergochip; the intestinal microbiota composition - by bacteriological method. Results Complementary foods were introduced to children of both groups at the age of 5.5 [5–6] months old. At 13–18 months children received 10–12 groups of products. Group I - M TIgE=54.0 kE/L. A moderate/high level of sensitization was detected in 25 children: egg (Gal d1, d2, d3) - 11, cow's milk protein (CMP) (Bos d6) – 5, cat (Fel d1, 7) – 4. Monosensitization was detected in 12 children: wheat, birch pollen, egg white, cat, CMP. 7 children had polyvalent sensitization to food, respiratory and epidermal allergens. Group II - M TIgE=105.3 kE/l. A moderate/high level of sensitization was detected in 6 children: CMP (Bos d4, Bos d5, Bos d8), egg (Gal d1, 2, 3), wheat, soy, potato, shrimp, mackerel, cod, cashew, walnut. Sensitization to respiratory allergens was detected in 6 children: birch pollen (Bet v1, Mal d1), cat (Fel d1, 4, 7), dog (Can f1, 4, 6), house dust mite. The following correlations were revealed: positive – Proteus spp. & TIgE; Proteus spp. & sIgE to Gal d1, d2, d3, cashew, cod (R=0,30, 0,22, 0,23, 0,38, 0,38, 0,38); Clostridium spp. & sIgE to sheep's milk, Gal d4, cashew, fenugreek, cod, Fel d1 (R=0,22, 0,22, 0,22, 0,30, 0,22, 0,23); negative - Bifidobacterium spp. & sIgE to Gal d2 (R= - 0,59). Conclusions The obtained results indicate association between Proteus spp. and Clostridium spp. of the intestinal microbiota with progrediency of atopy in the period of nutrition diversification.