Microbial degradation of chloropyrofos, profenophos and cypermethrin by multi-trait PGPR strains

Excessive use of pesticides contaminates the environment and causes adverse effect on living organisms. Multi-trait PGPR with pesticide degradation ability provides a significant advantage over the use of chemical fertilizers and removal of pesticides from the environment. In the present study, three multi-trait PGPR strains (RA4, RA11 and RA26) were used to check the pesticides (chloropyrofos, profenophos and cypermethrin) ability in liquid medium. The quantitative analysis of microbial degradation of pesticides was checked by using HPLC. Bacterial strain RA11 was degrading the profenofos, chloropyrofos and cypermethrin 94.5 %, 81.3 % and 67.7 % after 4 days of incubation in BSM medium. Bacterial strain RA26 was degrading profenofos 99.17 % after 4 days. Complete degradation (99.5 %) of chlorpyriphos was shown by strain RA4 after 4 days of incubation in BSM medium. Biofilm synthesis is beneficial for bio control of diseases and enhancing the nutrient availability to plants. All multi-trait PGPR strains (RA4, RA11 and RA26) were showing biofilm synthesis in the presence of pesticides (chloropyrofos, profenophos and cypermethrin). The maximum biofilm synthesis was shown by strain RA11 at 100 μg/ml concentration of cypermethrin. The multi-trait PGPR RA11 showed chemotactic behaviour against chlorpyrifos, cypermethrin and profenofos pesticides.