Combination of Honey and Nigella sativa Oil Reduce Oxidative Stress and Inflammation in Unilateral Ureteral Obstruction in Rats

Renal fibrosis plays a significant role in the development of chronic kidney disease (CKD). Therefore, treatment of renal fibrosis is essential in reducing the incidence of CKD-related problems. Due to their function as anti-inflammatory and antioxidant agents, honey and Nigella sativa may have therapeutic promise in renal fibrosis. This study aim to investigate the effects of the combination of honey and Nigella sativa oil as anti-inflammatory and antioxidant agents in a rat model of unilateral ureteral obstruction (UUO)-induced kidney injury. In this study, thirty male Wistar rats were divided randomly into five groups. One sham group, one UUO group, and three UUO groups that received varied doses of the combination of honey and Nigella sativa oil (HoNi). HoNi was administered to the rats orally, once daily for 21 days starting from the 14th day post-UUO induction. After treatment, blood samples were collected, and the levels of antioxidant enzymes; Superoxide Dismutase (SOD), Glutathione (GSH), and Catalase (CAT) were measured. The inflammatory marker; C-reactive protein (CRP) activity, and haemoglobin (Hb) levels were also measured. The results showed that the administration of the combination of honey and Nigella sativa for 21 days in UUO rat model significantly reduced CRP, while increasing the activities of SOD, GSH, and CAT, and also enhanced haemoglobin levels in a dose-dependent manner.These findings suggest that the renoprotective effect of the combination of honey and Nigella sativa oil maybe associated with its anti-inflammatory and antioxidant properties.