FIGURE 2 from Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers

PARP1 inhibition prevents ribosome biogenesis and decreases protein translation. Treatment with niraparib significantly decreases rRNA subunit (18S) production in endometrial (A) and ovarian (B) cancer cells as assayed by qPCR. Each bar represents the mean + SEM; n = 3. Bars marked with asterisks are significantly different; Student t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Puromycin incorporation is diminished with niraparib treatment in endometrial (C) and ovarian (D) cancer cells as assayed by Western blotting. E and F, Quantification of puromycin incorporation assay from C and D, respectively. Each bar represents the mean + SEM; n = 3. Bars marked with asterisks are significantly different; Student t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.