FIGURE 5 from Hemangiosarcoma Cells Promote Conserved Host-derived Hematopoietic Expansion

Gene expression analysis in DHSA-1426 hemangiosarcoma cells and xenograft tumors using RNA-seq data. A, The volcano plot visualizes 2,391 significant DEGs (1,034 upregulated and 1,357 downregulated) in DHSA-1426 cells (n = 3) compared with nonmalignant endothelial cells (n = 4; q-value < 0.01; log₂ fold change > |2|). The DEGs are indicated by red dots. NS = not significant; log₂FC = log₂ fold change. B, Gene Ontology enrichment analysis depicts biological processes associated with significant DEGs. P, adjust = adjusted P-value. C, Bar graphs present normalized gene expression values of CSF3, IL6, and IL8 in RNA-seq data generated from canine nonmalignant endothelial cells (n = 4) and DHSA-1426 hemangiosarcoma cells (n = 3). D, Schematic illustration visualizes the experimental steps for species-specific gene expression analysis of xenograft tumors using RNA-seq data. E, Bar graphs show the expression of mouse-specific genes in RNA-seq data generated from xenograft tumor tissues of canine hemangiosarcoma (n = 4) and mouse lymphoma (n = 7). A two-way ANOVA test was conducted to compare the means between groups. ****, P < 0.0001