FIGURE 4 from Hemangiosarcoma Cells Promote Conserved Host-derived Hematopoietic Expansion

Hematopoietic support and stromal regulation of canine hemangiosarcoma cells. A, Flow cytometric data depict populations of cells expressing CD43 and CD45 differentiated from CD34⁺ hUCB cells. CD34⁺ hUCB cells were pooled from 2 patients. M2-10B4, hBM-MSCs, and canine hemangiosarcoma cells (DHSA-1426 and EFB) were seeded on gelatin-coated 24-well plates at a density of 1 × 10⁵ cells/well. Gelatin-coated wells without stroma served as a negative control. Surface antigens CD34, CD43, and CD45 were analyzed at week 5. B and C, Bar graphs illustrate number of different colonies formed by hUCB CD34⁺ cells cocultured with feeder cells. Both DHSA-1426 and EFB canine hemangiosarcoma cell lines expanded hUCB CD34⁺ cells similarly to the M2-10B4 and hBM-MSC positive control lines, while gelatin-coated wells alone failed to support expansion. Burst-forming unit-erythroid (BFU-E), CFU (colony-forming unit)-Erythroid (CFU-E), CFU-granulocyte/macrophage (CFU-GM), CFU-macrophage (CFU-M), and CFU-granulocyte/erythroid/macrophage/megakaryocyte (CFU-GEMM) were determined for CFU assay.