Method Development and Validation of Levetiracetam By UHPLC in Bulk and Tablet Dosage Form

The new, rapid, sensitive, simple, precise and accurate Ultra High Performance Liquid Chromatography (UHPLC) method was development and validation of levetiracetam by UHPLC in bulk and tablet dosage form. The column used was Agilent C18 column (4.6mm x 100mm; 2.5µm) with mobile phase containing the MEOH: Water (0.1% TEA with PH 5.5 with OPA) in the ration of 20:80% v/v. The detection wavelength was 207nm and flow rate were 0.7ml/min. The retention time of levetiracetam were found to be 4.424min According to the ICH guidelines, linearity, accuracy, range, and robustness were all within acceptable limits.