FIGURE 6 from LYVE-1–expressing Macrophages Modulate the Hyaluronan-containing Extracellular Matrix in the Mammary Stroma and Contribute to Mammary Tumor Growth

Depletion of LYVE-1⁺ macrophages promotes a shift toward a proinflammatory environment. A, Volcano plots of four macrophage clusters with log(fold change) of Lyve1CreCsf1rfl/fl relative to Csf1rfl/fl and significance (P < 0.05) denoted as a red (upregulated) or blue (downregulated) coloration. B, Intracluster GSEA Lyve1CreCsf1rfl/fl relative to Csf1rfl/fl macrophage clusters (NES adjusted P-value < 0.05) with detailed gene set names in Supplementary Table S2. Quantification (C) and representative images (D) of EO771 tumors from Csf1rfl/fl and Lyve1CreCsf1rfl/fl mice immunostained for CD8a (green) and DAPI (blue). Each dot represents total CD8 count from one image, with five images taken per margin and five images taken per core of each tumor (n = 3). **, P < 0.01; ***, P < 0.001. Scale bars, 100 µm.