FIGURE 3 from LYVE-1–expressing Macrophages Modulate the Hyaluronan-containing Extracellular Matrix in the Mammary Stroma and Contribute to Mammary Tumor Growth

Genetic depletion of LYVE-1⁺ macrophages delays mammary tumor growth. A, Representative images from FFPE sections of EO771 tumors from Csf1rfl/fl (top) and Lyve1CreCsf1rfl/fl (bottom) mice stained by H&E, and immunostained for LYVE-1 (green), HABP (magenta), CD206 (red), and DAPI (blue; n = 5). B, EO771 tumor growth curve from Csf1rfl/fl (gray, n = 4) and Lyve1CreCsf1rfl/fl (blue, n = 4) mice, cutoff when the first mouse reaches endpoint. C, WNT-4226-65 L tumor growth curve from Csf1rfl/fl (gray, n = 4) and Lyve1CreCsf1rfl/fl (blue, n = 4) mice, cutoff when the first mouse reaches endpoint. D, Lung colonization of EO771 tumor cells measured by H&E staining. Colonization is quantified as percent colonization per lung by area, relative to experimental Csf1rfl/fl average. Quantification of TUNEL (E) and pHH3 (F) staining of EO771 tumors from Csf1rfl/fl (gray) and Lyve1CreCsf1rfl/fl (blue) mice from the core and margin. Representative images of TUNEL (G) and pHH3 (H) staining of EO771 tumors from Csf1rfl/fl and Lyve1CreCsf1rfl/fl mice from the core and margin. *, P < 0.05; **, P < 0.01. Scale bars, 100 µm. Each dot represents one mouse.