Preclinical evaluation demonstrates wide-field two-photon microscopy of amyloid-beta fibrils in Alzheimer's mouse brain tissue, indicating improved optical efficiency.
Single-molecule localization microscopy with temporal-focusing multiphoton excitation achieves wide-field and axially confined two-photon excitation of spontaneously blinking fluorophores, as reported by Fan-Ching Chien et al. in their Research Article (e202404942). This approach reduces the photobleaching of out-of-focus molecules to increase the utilization rate of the photon budget for exploring the three-dimensional morphology of fluorophore-labeled amyloid-beta fibril bundles in the brain tissues of a transgenic mouse with Alzheimer's disease.
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Lai et al. (2024) studied this question.
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