Protocol demonstrates acetaldehyde quantification in microbial fermentation samples, indicating a reliable approach for chemical monitoring.
This protocol details a broadly applicable approach for quantifying the concentration of acetaldehyde present in a microbial fermentation sample. To quantify acetaldehyde, a hydrazine derivatization reaction is performed following sampling using 2,4-dinitrophenylhydrazine (2,4-DNPH), which reacts with acetaldehyde in a 1:1 stoichiometric ratio to produce acetaldehyde-2,4-DNPH, a stable product at room temperature that can be quantified using high performance liquid chromatography (HPLC).
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Richardson et al. (2024) studied this question.
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