Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy v1

Steven P. Adams; Mason Mackey; Mark H. Ellisman

doi:10.17504/protocols.io.261ge5dpdg47/v1

May 17, 2024Open Access

Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy v1

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Abstract

This protocol details the labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy.

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Adams et al. (2024) studied this question.

synapsesocial.com/papers/68e69aefb6db6435876207b8 https://doi.org/10.17504/protocols.io.261ge5dpdg47/v1

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Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy v1

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Abstract

Discussion

Cite This Study

Labeling of microtubules using mouse anti-β-tubulin primary monoclonal antibody with secondary Fe-TAML-peg4-Cy5-goat anti-mouse IgG conjugate and oxidation of DAB with H2O2 for light and transmission electron microscopy v1

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Abstract

Discussion

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