FIGURE 5 from Mutant RAS-driven Secretome Causes Skeletal Muscle Defects in Breast Cancer

Molecular changes in plasma and skeletal muscles of mice bearing tumors initiated from transformed cell lines. A, Plasma miR-486 levels. B, Skeletal muscle miR-486 levels. C, Skeletal muscle Pax7 mRNA levels. D, Skeletal muscle Myh1 mRNA levels. E, Skeletal muscle p53 mRNA levels. F, Skeletal muscle Pdk4 mRNA levels. G, Skeletal muscle phosphorylated AKT levels. H, Total AKT levels. I, PTEN levels. J, Phosphorylated p-38 levels. K, Total p-38 levels. Quantification of Western blotting was performed using NIH ImageJ software 1.X. Note that in case of TKTB34-PIK3CA, the same blot was probed for AKT and pp38 and, therefore, one Ponceau S blot is shown. Similarly, the same TKTB34-RAS and TKTB6-RAS blot was probed with AKT and p-38 antibodies and one Ponceau S blot is shown.