FIGURE 6 from Mutant RAS-driven Secretome Causes Skeletal Muscle Defects in Breast Cancer

Cytokines/chemokines/growth factors secreted from cells transformed with mutant HRAS or PIK3CA. The assay was done twice in duplicate and intensity of the signals was normalized to internal controls in the array. Those with signals >5% of internal controls were considered for statistical analysis. A, Heat map of cytokines/chemokines/growth factors in array that compared data from TKTB34-RAS+TKTB6-RAS with TKTB34-PIK3CA. Statistically significant differences (P < 0.05) are shown on top. B, Same as in A except that comparison was limited to TKTB34-RAS with TKTB34-PIK3CA. C, Select cytokines/chemokines/growth factors differentially released by TKTB34-RAS and TKTB34-PIK3CA cells.