FIGURE 2 from Identification of MUC1-C as a Target for Suppressing Progression of Head and Neck Squamous Cell Carcinomas

MUC1-C regulates a global transcriptional program enriched for STAT/IRF signaling in HNSCC cells. A, RNA-seq was performed on CAL27/tet-MUC1shRNA and HSC3/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days. Volcano plots depicting downregulated (blue) and upregulated (red) DEGs (FDR<0.05; FC>2). Highlighted are the top DEGs by significance and magnitude. B, Common downregulated and upregulated genes in CAL27 and HSC3 cells with MUC1-C silencing. C, Purified chromatin from CAL27/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days was immunoblotted with antibodies against the indicated proteins. D and E, Candidate enrichment plots for the HALLMARK INTERFERON ALPHA RESPONSE (D), HALLMARK INTERFERON GAMMA RESPONSE (E) gene signatures. F and G, Box plots of selected ISG expression in CAL27 (F) and HSC3 (G) cells with MUC1-C silencing.