FIGURE 1 from Identification of MUC1-C as a Target for Suppressing Progression of Head and Neck Squamous Cell Carcinomas

Expression of MUC1 in HNSCC tumors and effects of silencing MUC1-C on HNSCC cell clonogenicity. A, Analysis of primary (P) and metastatic (M) HNSCC tissues for MUC1 expression using the GSE136037 dataset. B, CAL27/CshRNA and MUC1shRNA cells were analyzed for MUC1-C mRNA levels (left). The results (mean ± SD of four determinations) are expressed as relative levels compared with that obtained for CshRNA cells (assigned a value of 1; left). Lysates were immunoblotted with antibodies against the indicated proteins (right). C, The indicated CAL27 cells were analyzed for colony formation. Shown are representative photomicrographs of stained colonies (left). The results (mean ± SD of three determinations) are expressed as relative colony formation compared with that for CshRNA cells (assigned a value of 1; right). D, Amino acid sequence of the MUC1-C cytoplasmic domain (CD) highlighting direct interactions with JAK1 and STAT1. E and F, CAL27 cells expressing the indicated vectors were treated with vehicle or DOX for 7 days. Lysates were immunoblotted with antibodies against the indicated proteins (E). Cells were analyzed for colony formation (F). Shown are representative photomicrographs of stained colonies (top). The results (mean ± SD of three determinations) are expressed as relative colony formation compared with that for control cells (assigned a value of 1; bottom).