Green Silver Nanoparticles Synthesized fromMoringa OleiferaStem Bark Elicits Effective Antidiabetic Effect in Alloxan-Induced (Type I) Diabetic Wistar Rats

Abstract ID 92638 Poster Board 081 The use of nanomaterials in antidiabetic research is on the rise because of their special qualities, like their small size, biocompatibility, and capacity to penetrate cell membranes for drug delivery. In this study, the efficacy of an aqueous extract of Moringa Oleifera (MoSB) in crude and nanosized form using silver nanoparticles was observed. MoSB and its nanoparticles (MoSB-AgNPs) were prepared according to standard procedures and their effect was assessed on relevant enzymes associated with diabetes. Male Wistar rats were randomly separated into 7 groups of five rats each. The induction of diabetes in rats was by a single intraperitoneal injection of alloxan (180 mg/kg body weight). Rats were grouped as follows: Group 1 (normal Control); Group 2: (diabetic control); Group 3 (diabetic + 15 mg/kg nanosized extract); Group 4 (non-diabetic + 15mg/kg nanosized extract); Group 5 (non-diabetic + 250mg/kg aqueous extract); group 6 (diabetic+250mg/kg aqueous extract); group 7(diabetic+ 5mg/kg Glibenclamide (standard drug). The experiment lasted for 21 days. The results revealed that the blood glucose levels were significantly lower (P< 0.05) in rats administered MoSB-AgNPs when compared with the diabetic control group. Furthermore, administration of MoSB extracts and MoSB-AgNPs attenuated oxidative stress by decreasing MDA levels, enhancing superoxide dismutase, catalase and reduced glutathione activities, reinstated the lipid profiles and liver marker enzymes to nearly normal levels and restored pancreatic histological integrity in diabetic rats. The results reveal that M. Oleifera and MoSB-AgNPs exert their antidiabetic effects through the modulation of glycemic and atherogenic indices as well as the mitigation of free-radical-mediated damages. The present findings revealed that plant-mediated silver nanoparticles significantly improved the alloxan-induced diabetic changes in various treated rats and might be used in the treatment/ management of diabetes. The Authors DO NOT Recieve any Funding from Local, National and International Bodies. The Research is self funded.