Chlorophytum alismifoliumBaker Ameliorates Hyperglycaemia: Correlation Between Blood Glucose Levels and Some Biomarkers

Abstract ID 100781 Poster Board 487 Introduction: Medicinal plants are widely utilized in the management of many ailments. Chlorophytumalismifolium Baker ameliorates diabetes mellitus and its complications. This study focused on the antihyperglycaemic effect of C. alismifolium and the correlation of blood glucose level with some biomarkers using the Enzyme-linked Immunosorbent Assay (ELISA) technique. Method: Diabetes was induced in the rats using streptozotocin. The rats were administered graded doses (150, 300 and 600 mg/kg) of the various extracts and fraction of C. alismifolium daily for 28 days. Blood glucose was measured weekly and serum dipeptidyl peptidase 4, (DPP-4) and peroxisome proliferator activated receptor gamma (PPAR- ɤ) levels were evaluated at the end of the study using ELISA technique. (SPSS) Version 20 was used for the analyses. Data were expressed as Mean ± Standard Error of the Mean (S.E.M.) and the differences between means were analyzed using One way and Repeated Measure ANOVA followed by Bonferoni's post hoc. Pearson's correlation analysis was performed to measure the association of blood glucose levels on day 28 with serum PPAR- ɤ, AR and DPP-4. Values of P≤ 0.05 were considered statistically significant. Results: Induction of diabetes significantly (p<0.001) raised the blood glucose level in the hyperglycaemic rats compared to the normal control. Administration of the various extracts and fraction of C. alismifolium significantly (p<0.05) lowered the blood glucose levels compared to the hyperglycaemic control and over time. Ethylacetate extract of C. alismifolium (EACA) produced the best glycaemic control and significantly (p<0.05) increased PPAR- ɤ expression and markedly (p<0.05) decreased serum levels of DPP-4. Pearson's correlation analysis revealed a significant negative correlation (p<0.001) between the blood glucose levels and PPAR-γ. However, a significant positive correlation was observed between the blood glucose levels and DPP-4 (p<0.001). Conclusion: EACA elicits antihyperglycaemic activity which is possibly mediated through increased PPAR- ɤ expression and decreased serum level of DPP-4. The authors will appreciate funding from ASPET.