Bacteriological Profile and Antibiogram of Aerobic Bacteria Isolated From Patients With Ventilator Associated Pneumonia Admitted in Critical Care Unit of a Medical College in West Bengal

Culture sensitivity of deep tracheal suction samples collected from intubated patients has paramount importance in getting a proper antibiogram which can prevent morbidity and mortality and reduce the duration of ICU stay of the patients. Objectives are to nd out the bacteriological prole of isolates from deep tracheal suction samples at zero hours and at 48 hours of intubation, along with their antibiotic sensitivity pattern. 51 participants whose samples showed no growth at zero hours of intubation were included. Among the remaining 51 samples, 36 were found culture positive at 48 hours of intubation, where male participants were the majority with >60 years age group being maximally affected. Samples were collected in ICU, sent immediately to the microbiology laboratory followed by direct gram staining, and after plating, incubation, motility test and various biochemical identication methods, ABST was performed. Incidence of causative bacteria revealed gram negative bacilli to be more than gram positive organisms. Klebsiella pneumoniae showed maximum sensitivity to amikacin with other drugs being partially sensitive. Acinetobacter baumannii complex showed maximum sensitivity to levooxacin and amikacin, while being completely resistant to all other drugs. Amikacin and piperacillin tazobactam served the best combination therapy against Pseudomonas aeruginosa, while MRSA showed maximum sensitivity to linezolid. Antibiogram is thus an important tool in choosing selected antibiotics in order to prevent emergence of bacterial resistance in ventilator patients.