AB154. SOH24AB_261. Introducing the first, high fidelity, tissue and organ-level digital reconstruction of the in vivo human abdomen

Background: The Visible Human Project is a public domain library of cross-sectional cryosections obtained from female and male cadavers. Here, we explore the use of one such dataset—the male Korean human [Visible Korean Project (VKP)] and how it can be utilised to generate high fidelity digital reconstructions of the human abdomen. Methods: The VKP male cadaver dataset contains used 5,960 anatomical axial sections. Each was downloaded in JPG format and imported into HOROS© using JPG to DICOM plug-in. For each abdominal organ, regions of interest (ROI) were manually isolated using the pen function. The 'generate missing ROI' tool was used for large indiscriminate areas of data. Once ROIs were complete, a pixel value of 0 was set for outside the ROIS. ROIs were exported and saved. Once all ROIs of a particular organ/feature were generated they were reimported into the master data set. The master set was in black and white and pixel values within the ROIs were manipulated to 300 in order to create 'white' three-dimensional surface renders of each feature. These were then exported as an STL file, into Cinema4D©, for post-processing and final rendering. Results: The methodology enabled generation of a complete, high fidelity, reference data set of abdominal organs and both mesenteric and non-mesenteric domains. The combined dataset is an organ-level digital reference for the in vivo human abdomen. The methodology was extended to the reconstruction of tissue-level anatomy (i.e., to blood vessels, lymphatic and nervous structures). The final combination is the first tissue and organ level digital reconstruction of the in vivo human abdomen. Conclusions: A methodology was developed to generate the first, high fidelity, tissue and organ-level reconstruction of the in vivo human abdomen. The methodology provides a reference of normality and can be extended to the disease setting.