Quantitative Analysis and Antimicrobial Efficacy of Juice and Their Extracts from Citrus Sps. Against Selected Microorganisms

Antimicrobial efficiency of Citrus limon Burn.and Citrus medica L. different juice concentration and juice extract were examined by solvent fractionation partition extraction using n-Hexane and ethyl acetate as solvents and tested against six gram positive and six gram negative bacteria and five fungi by agar well diffusion method using positive control.The Citrus limon exhibited antimicrobial activities with zones of inhibition ranging from 7 to 45mm, 7 to 20mm, 7 to 21mm and 7 to 18mm for fresh juice and Juice: DMSO (1:1), n-Hexane, Ethyl acetate and D/W extracts respectively.Similarly Citrus medica displayed antimicrobial activities with zones of inhibition ranging from 7 to 27mm, 7 to 24mm, 7 to 25mm and 7to 19mm for fresh juice, Juice: DMSO (1:1) and n-Hexane, Ethyl acetate and D/W extracts respectively.MIC and MBC value ranging from 0.125mg/ml to 4mg/ml for different juice concentration and 4mg/ml to >8mg/ml found in different juice extract.n-Hexane (16mm) and ethyl acetate (18mm) juice extract of Citrus medica exhibited good zone of inhibition against Aspergillus niger and both Citrus sps.showed presences of phenolic content range from (3.81mg/ml-5.02mg/ml) and flavonoid content (3.09mg/ml-7.28mg/ml).These results encourage the identification of active substances which could be used as lead(s) molecules in development of new antimicrobial drugs.