April 27, 2024Nature CommunicationsOpen Access

Efficient gene knockout and genetic interaction screening using the in4mer CRISPR/Cas12a multiplex knockout platform

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Scalable screening of genetic interactions and paralog synthetic lethals is achieved using the newly developed multiplex in4mer Cas12a knockout platform.
A fivefold reduction in library size is attained compared to existing methods, while targeting approximately 4000 paralog pairs in genome-scale assays.
Screens in cancer cells using arrays of four guide RNAs reliably distinguish essential genes, supporting broader adoption of combinatorial knockout assays.

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Cell-based screening demonstrates robust multiplex gene knockout and synthetic lethal mapping in cancer cells, highlighting reduced assay costs.

Anvar et al. (2024) studied this question.

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Cell-based screening demonstrates robust multiplex gene knockout and synthetic lethal mapping in cancer cells, highlighting reduced assay costs.