Application of the PCR Method to Detect Dna of the Causative Agent of Cystic Echinococcosis in the Blood

Echinococcosis remains a complex multidisciplinary problem. Due to frequent intra- and postoperative complications, and distant recurrence of echinococcosis, the successful treatment of the patient depends on interaction of doctors in surgery and therapeutics. To date, there has been no consensus in the Russian Federation on management tactics of echinococcosis patients. There are no protocols or clinical guidelines to choose a surgical intervention volume and method or the duration of anti-relapse treatment courses. Currently, the main laboratory method to evaluate the effectiveness of surgical and anti-relapse treatment is enzyme immunoassay (ELISA) to detect IgG responses to unilocular echinococcus antigens. During cyst growth, antibody titers can vary greatly and initiate cross-reactions. Therefore, a search remains relevant for diagnostic methods that allow assessing infection recurrence risks to determine the anthelmintic therapy duration. The PCR method using our developed primer pairs, isolation conditions, and the amplification mode, studied hydatid cyst fluid obtained by the PAIR with anaphylactic shock as a complication, as well as matched blood samples of the patient taken within 12 hours after anaphylactic shock and a month after anthelmintic therapy. Echinococcalspecific DNA was found by PCR in the cyst contents and the blood samples of the patient obtained during the surgical intervention and a month after the antirelapse treatment. The molecular genetic research method can serve as a tool in the cyst echinococcosis diagnosis as an additional method to the histological one. Contamination of blood and other cavities and organs during the intervention is of great importance for determining a further management tactics of echinococcosis patients.