Cells stably expressing shRNA against MYO10 display altered cell motility.

Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10's cellular roles, we generated MYO10 knockdown (MYO10 KD ) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10 KD lines showed reduced proliferation and impaired cell migration in wound assays. Additionally, there were fewer edge filopodia in HeLa cells. Furthermore, MYO10 KD cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10's importance in cell proliferation, adhesion, and migration. These MYO10 KD lines provide an accessible cell culture model for future study of MYO10.