FIGURE 5 from Epithelial Expressed B7-H4 Drives Differential Immunotherapy Response in Murine and Human Breast Cancer

Anti-PD-L1 did not induce a proinflammatory immune response in B7-H4⁺ tumors. CD45⁺ cells were sorted from EMT6 tumors ± B7-H4 and subjected to NanoString gene expression analysis using the Mouse Pan-Cancer Immune Panel. A, Differentially expressed genes from CD45⁺ sorted cells from EMT6 control tumors when treated with anti-PD-L1 or isotype control and harvested 7 days after treatment. B, Changes in immune gene sets between isotype and anti-PD-L1–treated tumors. C, Differentially expressed genes from CD45⁺ sorted cells from EMT6-B7-H4⁺ tumors when treated with anti-PD-L1 or isotype control and harvested 7 days after treatment. D, Changes in immune gene sets between treatment groups of EMT6-B7-H4⁺ tumors. Data were analyzed by Wilcoxon rank-sum test. Genes with log₂ fold change >0.5 or <−0.5 and P-value <0.01 were regarded as significant. n = 6 mice per group for all groups.