Figure S5 from Epithelial Expressed B7-H4 Drives Differential Immunotherapy Response in Murine and Human Breast Cancer

Supplemental Figure 5. CD45+ gene expression changes from early and later tumor stage between EMT6 control and B7-H4+ tumors. (A) CD45+ cells were isolated from EMT6 control or B7-H4+ tumors 7 days post treatment with isotype control antibody. Differentially expressed genes are shown. n = 6 mice per group. (B) Macrophage function was upregulated in B7-H4+ tumors, but minimal other differences were seen. (C) Differentially expressed genes between control and B7-H4+ tumors at a later stage. n = 12 mice per group. CD45+ cells were harvested when tumors reached 500mm3. (D) Macrophage function remains elevated in B7-H4+ tumors, but minimal differences were detected. Data were analyzed by Wilcoxon rank sum test. Genes with log2 fold change >0.5 or <-0.5 and p-value <0.01 were regarded as significant.