Figure S2 from Epithelial Expressed B7-H4 Drives Differential Immunotherapy Response in Murine and Human Breast Cancer

Supplemental Figure 2. B7-H4 expression is not affected by type I or II interferon or TGF-β treatment in vitro. MMTV-neu epithelial cells that have high levels of endogenous B7-H4 were treated for 72 hours with IFNα or IFNγ at 100ng/mL. B7-H4 expression was analyzed by flow cytometry (n=4-5 per group). Similarly, B7-H4 expression was not altered by TGF-β expression (10 ng/mL) in vitro after 72 hours. Data were analyzed by One-way ANOVA or unpaired t-test.