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April 22, 2024Open Access

Cell culture of THP-1 monocytes and differentiation into macrophages with PMA v1

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Authors

SOSylvia Oghogho OmageMWMaria WallertSLStefan Lorkowski

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Overview

Laboratory protocol demonstrates culture of THP-1 monocytes and PMA differentiation into macrophages, highlighting standardized methods for immune assays.

Key Points

  • Culturing THP-1 monocytes and inducing differentiation into adherent macrophages with phorbol 12-myristate 13-acetate provides a reliable model for downstream immunological assays.
  • Displaying a doubling time of 35 to 50 hours, these human cells originate from acute monocytic leukemia and maintain viral safety while growing in standard suspension culture.
  • Protocol steps establish chemical differentiation via phorbol 12-myristate 13-acetate, supporting lipid metabolism studies while noting sensitivity to baseline cell culture density.

Cite This Study

Omage et al. (2024) studied this question.

synapsesocial.com/papers/68e6e3dbb6db64358765efaahttps://doi.org/10.17504/protocols.io.5jyl8255dl2w/v1
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  1. 1An Optimized Protocol for the Differentiation and Fibrosis-Associated Transcriptional Priming of THP-1 Macrophages2026
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