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May 2, 2026SHILAP Revista de lepidopterologíaOpen Access

The baseline levels of receptors and cytokines in phorbol 12-myristate 13-acetate-induced THP-1 monocyte to macrophage cells

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Authors

SWSri WulandariTSTri Agusti SholikahSJSinu Andhi Jusup

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Overview

Randomized trial evaluates pro-inflammatory receptor and cytokine expression in THP-1 macrophages, suggesting implications for inflammation research.

Key Points

  • This study aims to assess the baseline expression of pro-inflammatory receptors and cytokines in PMA-induced THP-1-derived macrophages compared to monocytes.
  • PMA-treated THP-1 cells were evaluated for mRNA levels of TLR4, RAGE, and TLR7 using RT-qPCR.
  • Cytokine levels (IL-6, TNF-α, IL-18) were quantified using ELISA in culture supernatants.
  • Comparative analysis was performed between THP-1-derived macrophage-like cells and their monocyte precursors.
  • mRNA expression of TLR4, RAGE, and TLR7 increased twofold in PMA-induced cells compared to monocytes.
  • IL-6 levels were significantly elevated (p = 0.0277) in THP-1-derived macrophages.
  • TNF-α levels also showed significant increase (p = 0.0105), while IL-18 levels were not significantly different.

Cite This Study

Wulandari et al. (2026) studied this question.

synapsesocial.com/papers/69f5945c71405d493afff362https://doi.org/10.33086/ijmlst.v8i1.7917
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