Establishment of a tumor-informed cfDNA Methylation Signature for head and neck Squamous Cell Carcinoma (HNSCC)

Introduction As translational methods improve, liquid biopsies are becoming increasingly relevant in early detection, therapy monitoring, and tumor surveillance. CfDNA has been proposed as a potential analyte, however clinical translation has been limited by cost and technological constraints. Here, we evaluate a novel technology of reduced representation bisulfite sequencing for investigation of cfDNA methylation in HNSCC.