Mp59-03 Exercise Improves Nocturnal Polyuria via an Increase in Nitric Oxide Production and Suppression of Intrarenal Renin-Angiotensin System Activity in Aged Mice

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP59)1 May 2024MP59-03 EXERCISE IMPROVES NOCTURNAL POLYURIA VIA AN INCREASE IN NITRIC OXIDE PRODUCTION AND SUPPRESSION OF INTRARENAL RENIN-ANGIOTENSIN SYSTEM ACTIVITY IN AGED MICE Takahiro Imanaka, Kentaro Takezawa, Hiroaki Kitakaze, Go Tsujimura, Sohei Kuribayashi, Norichika Ueda, Shinichiro Fukuhara, and Norio Nonomura Takahiro ImanakaTakahiro Imanaka , Kentaro TakezawaKentaro Takezawa , Hiroaki KitakazeHiroaki Kitakaze , Go TsujimuraGo Tsujimura , Sohei KuribayashiSohei Kuribayashi , Norichika UedaNorichika Ueda , Shinichiro FukuharaShinichiro Fukuhara , and Norio NonomuraNorio Nonomura View All Author Informationhttps://doi.org/10.1097/01.JU.0001009476.18935.3b.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Recently, we have reported that decreased nitric oxide (NO) production leads to intrarenal renin-angiotensin system (RAS) activation and salt-induced nocturnal polyuria (NP). We hypothesized that an increase in NO production can improve salt-induced NP. The aim of this study was to elucidate the effects of exercise, which increases NO production, on salt-induced NP and intrarenal RAS. METHODS: First, we evaluated the effects of exercise on NO production, NP and intrarenal RAS. We used 80-week-old C57/BL6 male mice. Mice were given 1% high salt diet (1%HSD) for two weeks and were randomized into the Exercise (Ex) or the Sedentary (Sed) group. Mice in the Ex group underwent moderate-intensity treadmill exercise for four weeks, while mice in the Sed group listened to the sound of the treadmill running. We evaluated 24 h urinary NOx, diurnal polyuria index (DPi), renal angiotensinogen (AGT), sodium chloride co-transporter (NCC) activity, and sodium excretion kinetics. Next, we evaluated the effects of NO suppression on exercise-induced changes in DPi. Mice were given 1% HSD and Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) and underwent treadmill exercise for four weeks (Ex with L-NAME group). We evaluated 24 h urinary NOx and DPi. RESULTS: The 24 h urinary NOx of the Ex group was significantly higher than that of the Sed group (p=0.05, Figure 1a). The DPi of the Ex group decreased significantly compared to that of the Sed group (p=0.01, Figure 1b). The renal AGT level and NCC activity of the Ex group were significantly lower than those of the Sed group (p=0.01 and p=0.02, respectively). The sodium excretion during the active period of the Ex group was significantly higher than that of the Sed group (p=0.02). The 24 h urinary NOx of the Ex with L-NAME group was significantly lower than that of the Ex group (p=0.01, Figure 1c). The decrease in DPi observed in the Ex group was abolished in the Ex with L-NAME group (p=0.05, Figure 1d). These findings indicate that the exercise-induced decrease in DPi is contributed by an increase in NO production. CONCLUSIONS: Moderate-intensity exercise increased NO production, suppressed intrarenal RAS, and improved salt-induced NP in aged mice, suggesting that an increase in NO production is a new treatment strategy for NP in the elderly. Download PPT Source of Funding: This research was supported by Grant-in-Aid for Scientific Research (C) (grant number: 23K08756) © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e957 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Takahiro Imanaka More articles by this author Kentaro Takezawa More articles by this author Hiroaki Kitakaze More articles by this author Go Tsujimura More articles by this author Sohei Kuribayashi More articles by this author Norichika Ueda More articles by this author Shinichiro Fukuhara More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...