Mp63-19 Non-Destructive Urinary Stones Analysis Utilizing Micro-X-Ray Fluorescence and Electron Microprobe Analysis: A Proof-of-Concept Study

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology (MP63)1 May 2024MP63-19 NON-DESTRUCTIVE URINARY STONES ANALYSIS UTILIZING MICRO-X-RAY FLUORESCENCE AND ELECTRON MICROPROBE ANALYSIS: A PROOF-OF-CONCEPT STUDY Mario Basulto-Martínez, Lauren Stone, Tariq Alotaibi, Jeremy Burton, Hassan Razvi, and Jennifer Bjazevic Mario Basulto-MartínezMario Basulto-Martínez , Lauren StoneLauren Stone , Tariq AlotaibiTariq Alotaibi , Jeremy BurtonJeremy Burton , Hassan RazviHassan Razvi , and Jennifer BjazevicJennifer Bjazevic View All Author Informationhttps://doi.org/10.1097/01.JU.0001009436.52988.91.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Urinary stone composition should be investigated whenever feasible to target prevention strategies and decreased stone recurrences rates. However, modern techniques for urinary stone analysis are typically limited to mass spectrometry, spectroscopy, and powder X-ray diffraction. Although efficient, these techniques require pulverization prior to analysis, preventing investigation of the internal heterogeneity exhibited by some stones. Therefore, we aimed to utilize non-destructive methods of spectrochemical analysis to determine the spatial distribution of elements within the stones at the micron scale and gain a greater understanding of the underlying mechanisms of stone formation. METHODS: Five human kidney stones were embedded in resin, cross-sectioned, and polished prior to geochemical analyses via two techniques: micro-X-ray fluorescence (µXRF), and electron microprobe analysis (EPMA). Both techniques used highly restricted excitation beams to determine the elemental composition of micron-scale "spots." Overlapping spots were scanned until the full sample surface had been covered, allowing for the generation of high-resolution maps of elemental/mineralogical distribution within the stones. µXRF was conducted with the Bruker M4 Tornado and the JEOL JXA-8530F microprobe instrument for EPMA. All analyses were carried out at the Earth and Planetary Materials Analysis Laboratory. RESULTS: An analysis of five stones revealed their respective bulk compositions to be as follows: 1, calcium phosphate (CaPO); 2, calcium oxalate (CaOx) with a cysteine band; 3, uric acid (UA) with CaOx bands; 4, CaOx with CaPO at surface; and 5, UA with CaPO and CaOx bands and amorphous inclusions. Moreover, spatially-resolved elemental identification was achieved, allowing for compositional mapping of the major types of stones, and locations of trace elements (e.g. Na, Zn, Cu) (Figure 1). CONCLUSIONS: This proof-of-concept study demonstrates the feasibility of utilizing non-destructive techniques for human urinary stone composition analyses using µXRF and EPMA, and allowed us to identify unique trace elements present within all five stones Further studies matching stone composition and patients' clinical data are warranted to better understand the underlying mechanisms of stone crystallization and how trace elements may impact this process. Download PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1038 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Mario Basulto-Martínez More articles by this author Lauren Stone More articles by this author Tariq Alotaibi More articles by this author Jeremy Burton More articles by this author Hassan Razvi More articles by this author Jennifer Bjazevic More articles by this author Expand All Advertisement PDF downloadLoading ...