Pd63-06 Exploring Bacterial Roles in Calcium Oxalate Stone Formation Through High-Resolution Imaging

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder II (PD63)1 May 2024PD63-06 EXPLORING BACTERIAL ROLES IN CALCIUM OXALATE STONE FORMATION THROUGH HIGH-RESOLUTION IMAGING Ava Mousavi, Reyhaneh Nazarian, William C. Schmidt, Karan Thaker, Javier Prieto, Gerard C. L. Wong, Aaron Celestian, Qian Chen, Jiahui Li, and Kymora B. Scotland Ava MousaviAva Mousavi , Reyhaneh NazarianReyhaneh Nazarian , William C. SchmidtWilliam C. Schmidt , Karan ThakerKaran Thaker , Javier PrietoJavier Prieto , Gerard C. L. WongGerard C. L. Wong , Aaron CelestianAaron Celestian , Qian ChenQian Chen , Jiahui LiJiahui Li , and Kymora B. ScotlandKymora B. Scotland View All Author Informationhttps://doi.org/10.1097/01.JU.0001009384.23104.ca.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Understanding the microbiological factors contributing to calcium kidney stone formation is vital for prevention. While it is well known that urease-producing bacteria contribute to struvite stone growth, it is less clear how bacteria may contribute to the development of other types of stones. Bacteria such as E. coli and P. aeruginosa have been detected in patient-derived calcium oxalate (CaOx) stones even in the absence of infection. Prior studies investigating the effect of bacteria on CaOx crystal growth have utilized pre-crystallized chunks, which are less likely to resemble physiological stone growth. Our study aims to elucidate the impact of bacteria on CaOx stone formation, through high-resolution imaging of de novo CaOx crystals in the presence of bacteria. METHODS: CaOx crystals were grown in vitro, using a novel method of de novo crystallization in a nonstatic in vitro environment that mimics the flow of urine. In separate experiments, CaOx crystals were grown in the presence of E. coli and P. aeruginosa with growth monitored for 3 and 10 days, respectively as compared to controls. RAMAN spectroscopy confirmed the chemical composition of crystals. Scanning Electron Microscopy (SEM) with energy dispersive spectroscopy was used to analyze crystal morphology and aggregation. Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) provided high resolution 3D crystal structure visualization. RESULTS: SEM revealed bacteria induced CaOx morphological changes and crystal aggregation in vitro, compared to controls (Figure 1). Further analysis with FIB-SEM revealed larger crystals with hollow internal structure versus solid controls (Figure 2). CONCLUSIONS: Complementary analytical methods demonstrate that biofilm-forming bacteria alter the morphology of CaOx crystals in an in vitro model. These morphological changes may promote crystal aggregation leading to accelerated stone growth. Further evaluation of this phenomenon in a 3D kidney model system will illuminate the effect of bacteria on CaOX crystal formation under physiological conditions. Download PPTDownload PPT Source of Funding: NIH/NIDDK K08DK13248601A NIH/NCATS KL2TR001882 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1294 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Ava Mousavi More articles by this author Reyhaneh Nazarian More articles by this author William C. Schmidt More articles by this author Karan Thaker More articles by this author Javier Prieto More articles by this author Gerard C. L. Wong More articles by this author Aaron Celestian More articles by this author Qian Chen More articles by this author Jiahui Li More articles by this author Kymora B. Scotland More articles by this author Expand All Advertisement PDF downloadLoading ...