Mp06-09 Multiplex Urinary Inflammatory Cytokine Analysis in Older Veterans With a History of Urethral Stricture Disease

You have accessJournal of UrologyReconstruction: Urethral Reconstruction (including stricture) I (MP06)1 May 2024MP06-09 MULTIPLEX URINARY INFLAMMATORY CYTOKINE ANALYSIS IN OLDER VETERANS WITH A HISTORY OF URETHRAL STRICTURE DISEASE Saager T. Chawla, Nolan Soutipan, Jad Shahan, Kyoko Sakamoto, and Mahadevan Rajasekaran Saager T. ChawlaSaager T. Chawla , Nolan SoutipanNolan Soutipan , Jad ShahanJad Shahan , Kyoko SakamotoKyoko Sakamoto , and Mahadevan RajasekaranMahadevan Rajasekaran View All Author Informationhttps://doi.org/10.1097/01.JU.0001009452.79331.fd.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Urethral Stricture Disease (USD) is highly prevalent among older male Veterans and the inflammatory microenvironment is recognized as an important causative factor. Although previous studies suggest a role for cytokines, the specific mechanisms by which elevated cytokine levels influence fibrogenesis is still unclear. Our objective is to evaluate inflammatory urinary cytokine levels in USD patients to discern a profile specific to USD patients and explore if these can be used as potential biomarkers to monitor disease progression as well as recurrence. METHODS: First catch urine samples were collected from USD patients (n=15) and 10 healthy controls (n=10), and 25uL samples were aliquoted for multiplex cytokine analysis. A Meso Sector Diagnostics (MSD) customizable U-Plex assay kit (CAT#: K15067M-1) was used to evaluate inflammatory cytokines (IFN-β, IFN-γ, IL-10, IL-1β, IL-2, IL-6, IL-8, MCP-1, TNF-α, TNF-β). Samples were simultaneously assayed for these 10 cytokines of interest with the MESO SECTOR S 600MM (MSD Maryland, USA). RESULTS: Our results showed that 5 out of 10 inflammation-related urinary cytokines (IL1-β, IL-6, IL-8, IL-10, TNF-β) were significantly elevated in USD patients (Figure 1; p<0.05). IL-8 was particularly high (over 150 folds when compared to controls). Increased IL-6 levels correlated with strictures diagnosed over 5 years ago (p=0.03). IL-8, IL-10, and TNF-β showed a significant correlation with history of prior transurethral interventions. Patients who underwent multiple procedures had significantly lower IL-10 and TNF-β, while patients who didn't receive any intervention had significantly lower values of IL-8. CONCLUSIONS: This is the first multiplex urinary cytokine analysis of USD patients, and our results indicate a relationship between each significant inflammatory urinary cytokine and urethral inflammation. Our findings suggest that urinary cytokine profile may serve as a non-invasive, sensitive, and specific biomarker that will enable diagnosis of USD occurrence and recurrence. Download PPT Source of Funding: Veterans Affairs Merit Award © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e55 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Saager T. Chawla More articles by this author Nolan Soutipan More articles by this author Jad Shahan More articles by this author Kyoko Sakamoto More articles by this author Mahadevan Rajasekaran More articles by this author Expand All Advertisement PDF downloadLoading ...