MP32-20 SPATIAL MIcroRNA EXPRESSION IN LICHEN SCLEROSUS-INDUCED URETHRAL STRICTURES

You have accessJournal of UrologyReconstruction: Urethral Reconstruction (Including Stricture) II (MP32)1 May 2024MP32-20 SPATIAL MIcroRNA EXPRESSION IN LICHEN SCLEROSUS-INDUCED URETHRAL STRICTURES Anna J. Saltman, Aaron Perecman, Travis Sullivan, Eric Burks, Myrtha Constant, Kimberly Rieger-Christ, and Alex Vanni Anna J. SaltmanAnna J. Saltman , Aaron PerecmanAaron Perecman , Travis SullivanTravis Sullivan , Eric BurksEric Burks , Myrtha ConstantMyrtha Constant , Kimberly Rieger-ChristKimberly Rieger-Christ , and Alex VanniAlex Vanni View All Author Informationhttps://doi.org/10.1097/01.JU.0001008816.80828.35.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Lichen sclerosus (LS) is an inflammatory disease of the genital skin that has been linked to urethral stricture disease (USD). LS strictures tend to be longer and are more likely to recur. Little is known about the underlying pathophysiology of this disease. MicroRNAs are small, non-coding segments of RNA that control gene expression by binding to mRNA. Previous studies have shown differential miRNA expression between LS and non-LS USD. We aim to use in situ hybridization (ISH) to evaluate spatial expression of these miRNA between LS and non-LS strictures. METHODS: Four miRNAs (miR-142, miR146a, miR-150, and miR155) were selected for analysis from a pilot study of 8 up-regulated miRNAs. ISH staining was done on tissue microarrays of 2mm punches consisting of 29 non-LS & 17 LS strictures. Five (5) micron sections were stained using heat induced epitope retrieval with miRNAscope HD reagents (ACDBio, Newark, CA). Mucosal and submucosal probe detection was scored using QuPath software. Continuous variables were evaluated using the Mann-Whitney U test and categorical variables were evaluated using the Chi-squared test, with SPSS v.28. RESULTS: There was a significant difference in the expression of submucosal miR-155 between LS and non-LS tissue (0.44 vs 0.07, respectively, p=0.002). No significant difference was seen for the submucosa of miR-142, miR-146a, miR-150, or the mucosal tissue for any of the miRNAs. A significant difference was seen in the number of patients that had lymphocyte-dense tissue between LS and non-LS submucosa (70.6% vs. 27.6%, respectively, p=0.005). CONCLUSIONS: In a previous study, utilizing qRT-PCR, we have shown that there is an overall up-regulation of miR-142, miR-146a, miR-150, and miR-155 in the tissue of LS strictures. With ISH we have shown that miR-155 has a differential spatial expression, prominent significantly in the submucosa. More research is needed to determine the significance of this up-regulation and determine its role in the diagnosis, prognosis, and therapy for LS strictures. Download PPT Source of Funding: Robert E Wise Research and Education FoundationEllison Foundation © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e522 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Anna J. Saltman More articles by this author Aaron Perecman More articles by this author Travis Sullivan More articles by this author Eric Burks More articles by this author Myrtha Constant More articles by this author Kimberly Rieger-Christ More articles by this author Alex Vanni More articles by this author Expand All Advertisement PDF downloadLoading ...