Figure 4 from Noninvasive Stratification of Colon Cancer by Multiplex PET Imaging

Imaging signatures depend on tumor context. A, The generation of mouse models. B, Transverse and coronal PET/MRI slices images showing [¹⁸F]FDG uptake in subcutaneous and orthotopic organoid models and GEMM of KrasG12D/+ Trp53fl/fl Rosa26N1icd/+ (KPN) colon cancer. KPN subcutaneous images reproduced here from Fig. 2B for comparison with KPN orthograft and GEMM. Tumors are outlined with a white dotted line. C, Standard uptake peak values (SUVpeak) PET quantification from images in B (n = 4 mice/model). Data compared using ANOVA followed by Fisher least significant difference test. D, Transverse and coronal PET/MRI slices images showing [¹⁸F]FDG uptake in subcutaneous and orthotopic KPN and Apcfl/+ KrasG12D/+ Trp53fl/fl Tgfbr1 fl/fl (AKPT) organoid models of colon cancer. Tumors are outlined with a white dotted line. KPN images reproduced here from Figs. 2B and 4B and for comparison to AKPT. E, SUVpeak quantification from images in panel D (Numbers inside bars show sample size, n). Data compared using two-way ANOVA, with the results of injection site shown. Details of all mice used in these studies are presented in Supplementary Table S1. F, Representative GLUT-1 immunohistochemistry of tumors from mice shown in panel E. Black scale bars represents 100 μm. G, H-score of GLUT-1 immunohistochemistry from mice shown in panel E. Box and whisker plots show range, median and interquartile range. Error bars in panel C and E represent standard deviation. Data compared using two-way ANOVA, with the results of injection site shown, * P < 0.05, ** P < 0.01, *** P < 0.001. (A, Created with BioRender.com.)