Phytochemical and chromatographical evaluation of Madanphala (Randia dumetorum Lamk.) from different Desha based on Akashadi Panchamahabhoota

Aims & Objective:- Evaluation of phytochemical and chromatographical constitution of Madanaphala, collected from different desha based on Akashadi mahabhoota. Madanaphala is the best vamana dravya. As per classics the best place to collect these vamana dravya is the land which is predominant of the Agni, Vayu and Akash mahabhoota. In this study we are going to evaluate the phytochemical and chromatographical qualities of the Madanaphala collected from Jangala desha (Kappatagudda) which is the ideal collection area mentioned by Acharya’s for vamana karma and the Madanaphala collected from the Anupa desha(Jamboti). Methodology:- 2 samples of Madanaphala, collected from the two different desha’s, one sample collected from Kappatagudda region and the other sample collected from Jamboti region. The collected fruits are dried, cleaned, powdered and were analyzed for phytochemical and chromatographical evaluation. Conclusion:- The phytochemical analysis of Madanaphala collected from two different region Kappatagudda and Jamboti didn’t show any changes. TLC of both the samples showed the presence of phytochemicals at different Rf values but the sample collected from Kappatagudda has more number of Rf values which indicate presence of more number of phytochemicals than in Madanaphala collected from Jamboti. The standard Rf value of Oleanolic acid is 0.57. In the HTPLC result the nearest Rf value observed was 0.51 which is found in the Madanaphala collected from Kappatagudda, but no nearest Rf value was observed in Madanaphala collected from Jamboti. So we can justify the reference which is explained by our Acharya Sushruta in the context of Bhumipravibhagiyamadhyaya that vamana dravya should be collected from desha predominant of Akash, Vayu and Agni mahabhoota. As our study also showed that Madanaphala collected from Kappatagudda region which is predominant of Agni, Vayu and Akash mahabhoota predominenet is of superior quality than the Madanaphala collectd from Jamboti region having Parthiva and Aap mahabhoota.