FIGURE 4 from Low Exposures to Amphibole or Serpentine Asbestos in Germline Bap1-mutant Mice Induce Mesothelioma Characterized by an Immunosuppressive Tumor Microenvironment

Triple indirect IF of crocidolite- and chrysotile-induced MMs from Bap1+/− mice. IF staining showing tumor cells as well as M1 and M2 macrophages in crocidolite- and chrysotile-induced MMs from 2 Bap1+/− mice. Histologic sections were subjected to triple indirect sequential IF using three different antibodies: WT1 to detect MM tumor cells (red circles; Red 555 fluorescence); CD11c to stain M1 macrophages (green circles; green 488 fluorescence); and CD163 marker to stain M2 macrophages (yellow circles; Cy5 yellow fluorescence).