FIGURE 2 from Low Exposures to Amphibole or Serpentine Asbestos in Germline Bap1-mutant Mice Induce Mesothelioma Characterized by an Immunosuppressive Tumor Microenvironment

TIME of crocidolite-induced MMs from Bap1+/− mice. A, Serial sections of a crocidolite-induced MM from Bap1-mutant mouse stained with several different immune cell markers: CD3 (T cells; arrows); CD45R (B cells); F4/80 (macrophages; arrows); and NK1.1 (natural killer cells), clockwise starting from top left. B, Serial sections showing distribution of macrophages in granuloma and TIME of MM from Bap1+/− mouse exposed to crocidolite. Left, Proinflammatory CD11c-positive M1 macrophages. Right, Protumorigenic CD206-positive M2 macrophages. Blue circles, granulomatous lesions; red squares, MM tumor areas. Note numerous crocidolite fibers (yellow block arrows) in granulomatous lesions.