Hairy root generation in common bean (Phaseolus vulgaris L.) and selection of Agrobacterium rhizogenes clones v2

The common bean (Phaseolus vulgaris L.) is one of the legumes used to study the molecular mechanisms that regulate mycorrhizal and rhizobial symbioses. To study these mechanisms, the generation of transgenic hairy roots is a very advantageous method for applying genetic approaches. However, the generation of hairy roots is a difficult task that requires a lot of skill and experience. Here we show a new version of an optimized protocol for the generation of hairy roots in common bean. This version includes photos and videos showing the procedure for preparing the Agrobacterium rhizogenes culture and the infection procedure, which were not included in the original version. Additionally, we included a step for growing hairy roots under hydroponic conditions.