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November 28, 2025GenesOpen Access

Optimizing an Ex Vitro RUBY-Equipped Method for Hairy Root Transformation of Peanuts: An Efficient Approach for the Functional Study of Genes in Peanut Roots

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Authors

XLXinyue LiJZJun ZhouFKFei Kong

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Implication

Observational analysis showed high-efficiency gene transformation in peanuts, indicating effective methods for studying root genes.

Key Points

  • High-efficiency gene transformation achieved with transformant frequencies from 46.2% to 73.7%.
  • Analysis using an expression vector with RUBY enabled direct visual selection of transformants effectively.
  • The method successfully overexpressed AhLRX6 in hairy roots, leading to thicker cell walls compared to the WT.
  • The findings may enable improved functional studies of genes in peanuts, enhancing agricultural applications.

Cite This Study

Li et al. (2025) studied this question.

synapsesocial.com/papers/6928f11ba65b730b9ea7a0c1https://doi.org/10.3390/genes16121401
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Optimizing an Ex Vitro RUBY-Equipped Method for Hairy Root Transformation of Peanuts: An Efficient Approach for the Functional Study of Genes in Peanut Roots2025 · 1 citations
  2. 2Enhancing Agrobacterium-Mediated Hairy-Root Transformation Efficiency in Peanut Through the Application of GRF, GIF and WOX Genes2026
  3. 3One‐Step ex Vitro Hairy Root Transformation of Soybean Using the RUBY Visible Reporter Gene2026
  4. 4Optimization of Hairy Root Transformation and Application of RUBY as a Reporter in Lotus corniculatus2024 · 11 citations
  5. 5An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion2024 · 84 citations