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September 10, 2025PlantsOpen Access

Establishment of an Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit

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This study demonstrates successful hairy root transformation in passion fruit, revealing implications for gene function analysis.

Transformation efficiencies of 11.3% were achieved using Agrobacterium rhizogenes strains, indicating significant progress.
Optimized conditions included OD600 = 0.6 and a 30 min infection duration, leading to enhanced transformation rates.
Utilizing the eGFP marker allowed for effective monitoring, while a red visual marker simplified identification of transgenic roots.
Overexpression of the PeMYB123 gene boosted proanthocyanidin content, showcasing the system's utility in functional genomics.

Pan et al. (2025) studied this question.

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