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April 3, 2026Gene ReportsOpen Access

A simplified and rapid hairy root transformation system for functional promoter and symbiosis studies in Fenugreek (Trigonella foenum-graecum)

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Develops an efficient transformation system in fenugreek, enabling effective functional genomics studies.

The aim is to establish a rapid hairy root transformation system for functional promoter analysis and symbiosis studies in fenugreek.
Developed a transformation protocol using Agrobacterium rhizogenes strains K599 and A4.
Compared transformation efficiency and root characteristics between K599 and A4 strains.
Validated transgene expression through fluorescence microscopy and PCR techniques.
Examined symbiotic competence by inoculating transgenic roots with Rhizophagus irregularis and Sinorhizobium meliloti.
K599 strain demonstrated a transformation efficiency of 92%, while A4 showed 58%.
Transgenic roots expressed eGFP and showed high DR5::GUS reporter activity at root tips.
Transgenic hairy roots maintained full symbiotic competence similar to wild-type roots.

Flores-Moreno et al. (2026) studied this question.

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