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This study aims to establish a genetic transformation system for Polygonatum cyrtonema using Agrobacterium rhizogenes, avoiding tissue culture procedures.

May 22, 2026Science of Traditional Chinese MedicineOpen Access

Establishment of an Agrobacterium rhizogenes-mediated genetic transformation system for Polygonatum cyrtonema

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Authors

BHBinjie HuangZhejiang A & F University CLChi LiuZhejiang A & F University JSJinping SiZhejiang A & F University

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Implication

Randomized trial demonstrates efficient genetic transformation in Polygonatum cyrtonema, suggesting new methods for enhancing medicinal plants.

Key Points

This study aims to establish a genetic transformation system for Polygonatum cyrtonema using Agrobacterium rhizogenes, avoiding tissue culture procedures.
Rhizomes from 2-year-old P. cyrtonema were transformed using A. rhizogenes strain K599 carrying the GFP gene.
An orthogonal experimental design optimized four factors: wounding, infection solution, inoculation, and co-culture duration.
Transformation efficiency was assessed via hairy root induction and confirmation through fluorescence microscopy, PCR, and quantitative PCR.
The optimal conditions yielded a positive transformation rate of 20.27%.
Distinct GFP fluorescence and PCR confirmed successful transgene integration.
Quantitative PCR analysis exhibited significant upregulation of GFP expression in transgenic hairy roots.

Cite This Study

Huang et al. (2026) studied this question.

synapsesocial.com/papers/6a0ff38cd674f7c03778c38e https://doi.org/10.1097/st9.0000000000000116

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Implication

Randomized trial demonstrates efficient genetic transformation in Polygonatum cyrtonema, suggesting new methods for enhancing medicinal plants.

Establishment of an Agrobacterium rhizogenes-mediated genetic transformation system for Polygonatum cyrtonema

Discussion

Member takes

Implication

Key Points

Cite This Study

Establishment of an Agrobacterium rhizogenes-mediated genetic transformation system for Polygonatum cyrtonema

Discussion

Member takes

Implication

Key Points

Cite This Study

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