What question did this study set out to answer?

The aim is to develop effective genetic transformation methods for Allium mongolicum to facilitate functional genomic studies.

June 13, 2026PlantsOpen Access

Establishment of Transient Expression and Hairy Root Induction Systems in Allium mongolicum

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Randomized trial establishes dual genetic transformation protocols in Allium mongolicum, implying enhanced research potential.

The aim is to develop effective genetic transformation methods for Allium mongolicum to facilitate functional genomic studies.
Established both in vivo transient expression and stable hairy root induction systems.
Identified vacuum impregnation as the optimal method for transient expression.
Optimized parameters using orthogonal experimental design for stable transformation.
Achieved stable integration and expression of transgene confirmed by GFP fluorescence and PCR analysis.
Utilized shortened stems as the optimal target explants for hairy root induction.
Localized candidate proteins AmJAZ2 and AmSWEET14 successfully to the nucleus and plasma membrane.

Cao et al. (2026) studied this question.

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