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August 22, 2026PlantsOpen Access

A RUBY-Assisted Hairy Root Transformation Platform for Metabolomic Profiling and Preliminary Functional Analysis of HpRHL76 in Herpetospermum pedunculosum

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Authors

SYShiyu YaoYYYujie YangJWJinyue Wang

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Overview

Experimental study demonstrates a high-efficiency visual transformation platform in Herpetospermum pedunculosum, facilitating functional genomics and metabolic engineering.

Key Points

  • Establish an efficient aseptic seedling and visual hairy root transformation system coupled with metabolomic profiling to enable functional genomics in Herpetospermum pedunculosum.
  • Developed a cotton-supported liquid culture method for rapid aseptic seedling germination.
  • Performed microsyringe-mediated stem-base inoculation using Rhizobium rhizogenes K599 carrying the visual reporter 35S::RUBY.
  • Conducted widely targeted metabolomics across wild-type, vector-free, and RUBY-expressing roots, followed by genome-wide identification and overexpression analysis of HpRHL-like bHLH genes.
  • Achieved rapid visual identification of transformed hairy roots with a maximum transformation efficiency of 93.33%.
  • Identified 242 metabolites with substantial shifts in amino acid, sugar, lipid, specialized metabolite, and hormone-related pathways.
  • Identified 80 HpRHL-like bHLH genes and confirmed that HpRHL76 overexpression increased mean root hair length from approximately 0.4 mm to 1.1 mm.

Cite This Study

Yao et al. (2026) studied this question.

synapsesocial.com/papers/6a895ffeca7ade938187ef24https://doi.org/10.3390/plants15162514
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  3. 3Traditional utilization, botany, phytochemistry, pharmacology, pharmaceutical analysis, processing and application of the seeds of Herpetospermum pedunculosum (Ser.) C.B. Clarke: a comprehensive review2024 · 2 citations
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