Supplementary Figure S6: (A) Fraction of immune vs. non-immune (primarily epithelial) cells from PyMT tumors used for RNA-seq. A small fraction of tumor tissues was isolated for this QC analysis, including CD45 fraction as presented in this figure. (B) Of the top 32 genes shown in Figure 3B, NE-specific genes were identified by performing qRT-PCR analysis in 10-week old PyMT NE+/+ (N=5) and PyMT NE-/- (N=4) pre-metastatic, malignant mammary tissues. 12 genes shown here were significantly different as a function of NE status. (C) Quantification of the total percentage of fibroblasts (CD140+ve cells) isolated from each genotype is indicated.
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Lulla et al. (2024) studied this question.
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