The effect of Propolis Nanoparticles irrigation on reduction of bacterial Endotoxins A Randomized Clinical Trial

Aim: The aim of this study was to evaluate the effect of propolis nanoparticles irrigating solution versus sodium hypochlorite on reduction of bacterial endotoxins.Materials and Methods: Forty-five single rooted non-vital permanent maxillary anterior teeth with tenderness to percussion and slight widening of lamina dura in medically free patients were included in the study. The patient's age ranged from 20-50 years, either males or females. Patients were randomly divided into three groups, 15 patients in each group, Group (1): 0.9% saline solution, Group (2): 2.5 % sodium hypochlorite (NaOCl) and Group (3): 20% Propolis nanoparticles. Two samples were taken for each group using paper points, one after access cavity preparation (S1) and the second sample after irrigation (S2). Samples were placed in a pyrogen free glass tube and stored at -20 degree Celsius then were assessed for quantification of bacterial endotoxins using ELISA kit, and subjected to statistical analysis.Results: Results showed that the use of 20% propolis nanoparticles as a root canal irrigant resulted in significantly higher reduction of endotoxins level compared to 2.5% NaOCl root canal irrigation.Conclusion: It could be concluded that the use of 20% propolis nanoparticles root canal irrigation allowed more reduction of endotoxins level significantly, compared to 2.5% NaOCl irrigation.