Some haematobiochemical alterations in buffaloes suffering from milk fever with trail of treatment

T his study was planned to investigate the effect of milk fever and treat- ment on haemato-biochemical parameters alterations in buffaloes.About 15, 4-5 years old buffaloes (5 healthy -10 suffering from milk fever) were divided into 3 groups (5/each).1 st group healthy buffaloes served as control, 2 nd group buffaloes suffering from slight milk fever or the first stage of milk fever (tremors in head and legs) and treated by 500 ml Calcium borogluconate (I/V) as one dose, along with 25 ml phosphonic acid/ buffalo (I/V), while the 3 rd group buffaloes were suffering from severe milk fever (depression, lateral recumbancy beside unconsciousness) and treated by 500 ml Calcium borogluconate (I/V) as one dose and 25 ml phosphonic acid (I/V)/buffaloe along with 1.5ml/50 kg bwt vitamin D (I/M) daily for 5 days.Blood samples were taken from each buffalo one pre and at 5 th days post treatment for determination of hematobiochemical parameters changes.Buffaloes suffering from milk fever revealed a significant reduction in calcium, phosphorus, α, β globulins, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GSH-px) beside an insignificant reduction in magnesium, potassium, red blood cells (RBCs), hemoglobin (Hb), packed cell volume (PCV), lymphocyte, basophil, monocyte, total protein, albumin , total globulin coupled with a significant increase in glucose, white blood cells (WBCs), neutrophil, eosinophil, cortisol, parathyroid, aspartate amino transferase ( AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), creatinin kinase (CK), creatinin phosphkinase (CPK), lactate dehydrogenase (LDH), and malondialdehyde (MDA) associated with significant increase in γ globulin.Treatment of diseased buffaloes using Calcium borogluconate, phosphonic acid and vitamin D improved its healthy status as well the hematobiochemical parameters and returned to nearly normal levels at 5 th day post treatment when compared with healthy control buffaloes.