Trimetazidine hydrochloride [1] (TMZ) is an antianginal drug (BCS Class I) used to treat angina pectoris.A simple, rapid, sensitive and economical RP-HPLC method were developed and validate (ICH M10) for quantification of trimetazidine in human plasma by using internal standard as caffeine.Liquid-Liquid extraction (LLE) method was developed for the optimum extraction of trimetazidine and caffeine from biological matrix.For that, Aliquots (450µL) of plasma were spiked with known amount of trimetazidine and caffeine.The separation was accomplished with the help of column Nucleosil ODS (15cm×4.6 mm, 5µ) at 25°C and an Agilent HPLC with control panel software.An isobestic wavelength set at 210 nm on advanced PDA detector and the mobile phase was composed of Methanol: Water: Acetonitrile: Sodium Hept-Sulfonate Buffer (40:60:10:03%, v/v/v/v).The injection volume was 5µL and the flow rate was 1.3 ml/min.
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Aa et al. (2024) studied this question.
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